application of molecular biology in disease diagnosis

In the smears of patients treated for OSCC, the percentage of apoptotic cells has been studied [28]. Ogden GR, Cowpe JG, Wight AJ: Oral exfoliative cytology: review of methods of assessment. Pitfalls in establishing a molecular diagnostic laboratory. Although tempting, broad-range assays aren’t always the best option for detecting multiple bacteria in a single sample, so pathologists must work with clinical colleagues to select and interpret the most appropriate tests. Photomicrograph of a brush biopsy specimen from oral submucousfibrosis showing anucleated hyperkeratinized cells and a superficial squamous cell. Acta Cytol. 10.1016/S0959-8049(03)00550-1. Then progesterone containing sample is added to this test tube which binds the antibodies forming antigen-antibody complex, whose concen­tration depends upon the amount of progesterone in the blood sample. Cowpe et al. Partridge M, Pateromchelakis S, Phillips E, Emilion GG, A'Hern RP, Langdon JD: A case control-study confirms that microsatellite assay can identify patients at risk of developing squamous cell carcinoma within field cancerization. The diagnosis of metastatic lesions usually is determined by fine-needle aspiration. Arch Otolaryngol Head Neck Surg. Edited by: García del Moral R. 1993, 245-63. | Cancer Res. 1991, 68: 1611-5. Cytopathol. Thanks to a simple, widely available technique – the polymerase chain reaction (PCR) – molecular techniques now serve a variety of applications in routine clinical laboratories, with real-time PCR allowing the rapid detection of various infectious microorganisms. Cancer. identified genetic alterations (LOH or MI) in all of the malignant lesions of the oral cavity included in their sample. Article  John, H.A., Birnstiel, M.L., Jones, K.W. For HSV lesions, in particular, the observation of the cytopathological features indicative of viral infections (binucleation, multinucleated cells) greatly improved with the liquid-based technique [26]. Cookies policy. Barany, F. (1991) Genetic disease detection and DNA amplification using cloned thermostable ligase. Bishop JW, Bigner SH, Colgan TH, Husain M, Howell LP, McIntosh K: Multicenter masked evaluation of AutoCyte Prep thin layers with matchedconventional smears–including initial biopsy results. Mashberg A, Feldman LJ: Clinical criteria for identifying early oral and oropharyngeal carcinoma: erythroplasia revisited. 5). In a study by the author comparing the post-radiation changes in normal and malignant oral cells it was found that various morphological abnormalities demonstrated a consistent significant increase with radiation dose [27]. Direct costs vary widely, depending on the test's complexity and sophistication. For HSV encephalitis, detection of HSV in cerebrospinal fluid (CSF) can direct specific therapy and eliminate other tests including brain biopsy. [55] used PCR techniques to amplify DNA from exfoliated cytology samples from oral carcinomas, for analysis of Restriction-Fragment Length Polymorphisms (RFLPs). In a recent study from Brazil [26] the liquid-based preparations resulted in higher specimen resolution as well as presenting a better cytological morphology for pemphigus vulgaris, squamous cell carcinomas, HSV lesions and fungus infections. Wahl, G.M., Stern, M., Stark, G.R. The clinical microbiology laboratory and infection control: Emerging pathogens, antimicrobial resistance, and new technology. Message not sent. [60] performed a microsatellite analysis of cells sampled from the oral cavity of oral and oropharyngeal cancer patients by exfoliative cytology and by mouthwash, finding LOH in 84% of samples, though with differences depending on tumour stage.

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