This Open Access article is distributed under the terms of the Creative Commons Attribution Noncommercial License ( of very dilute potassium permanganate solution to one of the test tubes of ethene and shake the tube a few times. 2a). Get Full Access Now We compared the morphology of the tissue specimens with the TOF profiles to ensure that high-quality tissue had been generated. Ultrasound time-of-flight (TOF) technology has been successfully used to monitor the critical processing step of tissue fixation with formalin. Therefore, we believe that this TOF ultrasound technology can provide histologic quality metrics and potentially be used for real-time tissue processing monitoring. When the test tubes have all been filled, loosen the retort stand and raise the apparatus so that the delivery tube no longer dips into the water. This processing step is a crucial part of FFPE tissue production, however, and with modifications to the TOF ultrasound instrument, the technology could be used to monitor wax infiltration as well, since the underlying principle of TOF change would still be present in wax. (Make sure the test tube is filled with water when you start to collect the gas produced.). All Rights Reserved. These gases which are produced are passed through sodium hydroxide solution to remove the carbon dioxide and sulphur dioxide produced from the side reactions. (e) Colon tissue average TOF trace with SD. FIG. The limewater should turn milky white, showing that the gas burns in air to produce carbon dioxide. Generally, if DNA degradation is the goal in an experimental setting, low frequency of 20–30 kHz is typical with high power settings.15 The high-frequency, low-intensity ultrasound in our monitoring technique is unlikely to generate significant DNA damage. The H-Beta-38 catalyst coated microplates exhibited the highest production rate of ethene. The variables for this experiment are: Material of Container: The beaker I keep the water in has to be the same as some materials are better conductors of heat than others. Fermenters are chambers in which microorganisms are cultured in a liquid or solid medium. Pour some ethanol into the boiling tube to a 2-3 cm depth. In this reaction the purple colour of the permanganate changes to colourless. The functional form of the TOF signal resulting from fluid exchange with the processing reagents was best fit with a single exponential (R2 adj = 0.99 ± 0.02; deviation from fit = 1.5% ± 1 of signal amplitude). The τ90 for the xylene incubation was 0.58 ± 0.10 hours for xylene and R2 of 0.982. Create one now! The journey toward this goal is fraught with challenges, not the least of which is summed up in the old computer science saying, “Garbage In, Garbage Out,” which in this case indicates that effective and accurate diagnostic tests require high-quality input tissue specimens. 2548, Word count: We monitored incomplete dehydration of tissue by skipping a key processing step, dehydration in absolute ethanol, and then correlated the τ90 with poor histomorphology, demonstrating that the technique can detect significant processing errors. D.R.B., A.T., D.C., and M.O. Whole slide images were obtained using an Aperio slide scanner. The current approach to solving the problem of preanalytical tissue processing errors, which is simply ensuring that all tissue processing steps last long enough so that even the most challenging tissue specimens have time to reach equilibrium with reagents, is unsatisfying for three reasons. Slides were developed using the OptiView DAB IHC Detection kit (Ventana Medical Systems, Inc.) and counterstained with hematoxylin. I will probably be using a copper container as it is easy to find and can withstand high temperature.
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